Using Antibodies in Microscopy: A Guide to Immunohistochemistry. Part 3: Post-Embedding Electron Microscopy Techniques

نویسندگان

چکیده

Abstract Immunohistochemistry (IHC) is the use of antibodies as probes to determine localization proteins in a cell or tissue sample. In microscopy, technique has been since 1940s by pathologists and research scientists. At first glance it appears straightforward procedure. practice, however, an IHC protocol many steps where problems can occur resulting either false positive negative data. be used for ultrastructural studies using electron microscopy (EM), but protocols light must adapted. While techniques have published extensively, certain amount confusion exists how optimize them. This series articles describes involved their function, some variations that specific on differing types. It hoped better understanding these will help guide users determining optimal conditions carrying through protocols.

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ژورنال

عنوان ژورنال: Microscopy Today

سال: 2023

ISSN: ['2150-3583', '1551-9295']

DOI: https://doi.org/10.1093/mictod/qaad050